KLOW Multi-Peptide Research Reagent

Heterogeneous Peptide Reagent Blend | 80mg Total Lyophilized Mass

Overview: The KLOW Blend is an 80mg multi-component peptide reagent combining four research-grade peptides. Designed specifically for complex regenerative biology assay design, its multi-pathway architecture enables the simultaneous in-vitro study of extracellular matrix remodeling, vascular signaling, cytoskeletal dynamics, and inflammatory pathway modulation.

Technical Specifications

Component Breakdown & Molecular Structure

The reagent is blended at a fixed molar ratio in a single lyophilization run to ensure single-batch homogeneity and strict reproducibility across experimental replicates.

1. GHK-Cu (50 mass units)

• CAS Registry: 89030-95-5 | PubChem CID: 378611

• Molecular Formula: $C_{14}H_{23}CuN_{6}O_{4}$

• Mechanism: Modulates extracellular matrix reconstruction via collagen I/III upregulation and glycosaminoglycan deposition.

• Structure: A naturally occurring copper complex where the $\text{Cu}^{2+}$ ion is coordinated by the imidazole nitrogen of histidine, forming a highly stable complex (log stability constant 16.44).

2. BPC-157 (10 mass units)

• CAS Registry: 137525-51-0 | PubChem CID: 9941957

• Molecular Formula: $C_{62}H_{98}N_{16}O_{22}$

• Mechanism: Activates endothelial nitric oxide synthase (eNOS) via the VEGFR2/Akt axis and modulates FAK/paxillin cytoskeletal signaling for endothelial cell migration and vascular tube formation.

3. TB-500 (10 mass units)

• CAS Registry: 77591-33-4 | Sequence Class: Acetylated Heptapeptide (Ac-LKKTETQ)

• Mechanism: Modulates G-actin sequestration and cytoskeletal organization. Its LKKTET actin-binding domain enables cell migration studies in scratch-wound assay models.

4. KPV (10 mass units)

• CAS Registry: 69630-60-0 | PubChem CID: 122130

• Mechanism: At nanomolar concentrations, blocks p65/RelA nuclear translocation via competitive importin-α3 binding, suppressing pro-inflammatory cytokine secretion independently of melanocortin receptor binding.

Comparative Research Context

The inclusion of KPV is the primary differentiator between KLOW and GLOW blends, providing direct control over inflammatory pathway interference during study conditions.

Quality Control & Sourcing (Profound Aminos)

• Sequence Integrity Verification: Beyond standard purity tests, each of the four distinct compounds is individually verified via Mass Spectrometry (LC-MS) to confirm molecular weight and sequence identity prior to batch release.

• Single-Batch Homogeneity: Blended at a fixed molar ratio (50:10:10:10) in a single run, eliminating the inter-batch variance that compromises assay reproducibility when pooling separate vials.

• Cold-Chain Integrity: Ships via a monitored cold-chain logistics protocol from a climate-controlled USA facility to prevent thermal degradation during transit.

Regulatory & Compliance Statement (RUO)

FOR RESEARCH USE ONLY. NOT FOR HUMAN OR ANIMAL APPLICATION.

• Regulatory Status: Research Use Only (RUO) under U.S. Federal Regulatory Framework.

• FDA Status: Not approved. All 4 components are FDA Category 2 bulk substances (Sept 2023). Not evaluated for safety or efficacy; not a drug, dietary supplement, or consumer product.

• Handling: Supplied solely for in-vitro testing and preclinical laboratory investigation. Gloves, lab coat, and protective eyewear are required during reconstitution and handling.

• Strict Prohibition: Promoting, distributing, or using this research reagent for any application involving administration to living organisms is strictly prohibited and violates U.S. federal law.

Frequently Asked Questions

• What does the 80mg format mean?

  It refers to the total lyophilized peptide content across all four components. The 50mg GHK-Cu loading ensures complete metal-ligand complexation across the reconstituted volume.

• How should KLOW be reconstituted?

  It is soluble in bacteriostatic water or sterile PBS buffer. Reconstitute slowly using a swirling motion (do not vortex). Aliquot immediately into working volumes and store at −20°C to avoid freeze-thaw degradation.

• Is a COA provided?

  Yes. Every batch includes a Certificate of Analysis verifying $\ge$99% purity per component via RP-HPLC, LC-MS identity confirmation, endotoxin screening (<0.25 EU/mg), lot number, and manufacturing date.

• Can KLOW be used outside a controlled laboratory?

  Absolutely not. Any non-laboratory use violates the Terms of Service.