5-Amino-1MQ (Research Grade)

Certified Reference Material (CRM)

Unlike peptide-based incretins, 5-Amino-1MQ is a Small Molecule Inhibitor targeting the cytosolic enzyme Nicotinamide N-methyltransferase (NNMT). It is engineered strictly for Cellular Metabolic Profiling, focusing on cellular bioenergetics, NAD+ flux quantification, and satellite cell proliferation in myofiber models.

Product Overview

• Product Format: Lyophilized Crystal/Powder (Vial)

• Grade: Reference Material (≥99% HPLC)

• Primary Application: 5-Amino-1MQ operates intracellularly by inhibiting NNMT. Research indicates that elevated NNMT activity is linked to compromised NAD+ availability and increased adiposity.

By blocking this enzymatic pathway in research models, 5-Amino-1MQ is utilized to study:

• Cellular Bioenergetics: Restoring the cytosolic NAD+/NADH ratio.

• Adipocyte Phenotypes: Investigating the reduction of lipogenesis (fat accumulation) without inducing cellular apoptosis.

• Myogenic Differentiation: Assessing markers in reversing sarcopenia (muscle wasting) in aged tissue models.

Technical Specifications

• Chemical Name: 5-amino-1-methylquinolinium iodide

• Chemical Class: Methylquinolinium Derivative (Small Molecule)

• CAS Number: 42464-96-0 (Iodide Salt)

• Molecular Formula: C₁₀H₁₁IN₂

• Molecular Weight: 286.11 g/mol (Salt Form)

• Purity: ≥99% (HPLC Verified)

• State: Crystalline Powder

• Solubility: Soluble in DMSO; Moderate in Water

Technical Stoichiometry Note: Most research protocols utilize the Iodide Salt form for stability. The cation alone has a molecular weight of ~159.21 g/mol, but the functional reagent supplied is the salt (MW 286.11 g/mol). Ensure calculations account for the salt weight during stoichiometry for accurate Molar concentrations in enzymatic assays.

Mechanism of Action: The “Methyl Sink” Blockade

1. NNMT Inhibition: The enzyme NNMT consumes Nicotinamide and S-Adenosylmethionine (SAMe) to produce 1-Methylnicotinamide (1-MNA). This process is often called the “Methyl Sink” because it drains the cell of energy precursors.

2. NAD+ Restoration: By inhibiting NNMT, 5-Amino-1MQ prevents this drainage, theoretically increasing the pool of NAD+ available for Sirtuin activation and mitochondrial function.

3. Adipocyte Bioenergetics: Cellular assays demonstrate that treated adipocytes show reduced volume and downregulated lipogenic gene expression.

Comparative Research Context

Practical Interpretation for Researchers:

• Select 5-Amino-1MQ for protocols focusing on cellular metabolism, muscle tissue preservation, and NAD+ pathways.

• Select Tirzepatide for incretin-based signaling models.

• Note: 5-Amino-1MQ is often studied in “Combination Protocols” alongside peptides but operates via a completely distinct, non-hormonal pathway.

Handling & Stability

• Storage: Store ideally at -20°C, or in a cool, dry place protected from light. The hygroscopic nature requires desiccation.

• Reconstitution: While soluble in water, DMSO is recommended for stock solutions to prevent precipitation in high-concentration assays.

• Safety Warning: Contains Iodide. May cause skin and eye irritation. Use strictly in a fume hood with appropriate PPE.

Why Researchers Source from PROFOUND AMINOS

1. Validated Salt Stoichiometry: Many grey-market vendors confuse the Cation MW (159.21) with the Salt MW (286.11), leading to massive titration variance in stock solutions. We explicitly label and supply the Iodide Salt form, ensuring accurate Molar concentrations.

2. USA Cold-Chain Integrity: While stable at room temperature, NNMT inhibitors can degrade under extreme heat during transit. We maintain a strictly monitored USA Supply Chain, shipping from climate-controlled facilities to preserve crystal lattice integrity.

3. “Post-GLP-1” Research Ready: As the market shifts from simple satiety peptides to complex metabolic modulators, our 5-Amino-1MQ is tested specifically for NAD+ Salvage Pathway activity.

Regulatory & Compliance Statement

Regulatory Classification: This product is strictly classified as a Chemical Reference Standard for Research Use Only (RUO). It is NOT a sterile pharmaceutical ingredient (API), dietary supplement, or drug intended for human or veterinary consumption. Any use involving bodily introduction (injection/ingestion) is strictly prohibited and violates federal compliance protocols.

FDA Warning: 5-Amino-1MQ is an unapproved new drug. The FDA has not evaluated it for safety or efficacy in humans. Selling or using this product for “Biohacking” or personal use is a violation of federal law.

Safety Standards (Iodide Salt): * H302: Harmful if swallowed.

• H315: Causes skin irritation.

• H319: Causes serious eye irritation.

• Protocol: Handle only in a laboratory setting with Nitrile gloves and safety goggles.

Frequently Asked Questions (FAQ)

Q: What is 5‑Amino‑1‑MQ and how does it work?

A: 5‑Amino‑1‑MQ is a small quaternary ammonium compound. In cell and animal studies, it acts as a selective inhibitor of nicotinamide N‑methyltransferase (NNMT). By blocking this enzyme, it increases cellular NAD+ and S‑adenosylmethionine levels, which influences energy metabolism.

Q: Is 5‑Amino‑1‑MQ a peptide?

A: No. Although some marketing websites erroneously call it a “peptide,” it is chemically a small molecule derived from quinoline.

Q: Is this product approved for human use or body composition improvement?

A: NO. This material is a lyophilized Chemical Reference Standard designed strictly for Isolated Cell Culture Protocols. It is not an FDA-approved drug or dietary supplement.

Q: What evidence supports its effects in animal models?

A: Pre‑clinical studies show promising preliminary effects. A 2017 study found that 5‑Amino‑1‑MQ reduced body weight and fat mass in obese mice. A 2024 study reported that aged sedentary mice given 5‑Amino‑1‑MQ experienced a ~40% improvement in grip strength. No comparable human data exist.

Q: What are the known safety concerns?

A: Safety data sheets classify the iodide salt as harmful if swallowed and irritating to the skin, eyes, and respiratory tract. Toxicology, long‑term effects, and appropriate dosing remain entirely unknown in humans.

Q: Why is the Molecular Weight listed as 286.11 g/mol?

A: We supply the Iodide Salt Form for enhanced stability. While the cation alone is ~159 g/mol, the salt form (286.11 g/mol) must be used for accurate Molar Titration calculations in enzymatic assays.

Q: Can this be used in combination with GLP-1 agonists in research?

A: Yes. Many protocols investigate “Dual-Pathway” models, combining a GLP-1 (for receptor signaling) with 5-Amino-1MQ (for intracellular bioenergetics) to observe distinct metabolic effects on adipocytes.